The short answer
Understand the system before choosing an action.
A checking account is a deposit account designed for frequent payments and money movement. Transactions may include deposits, debit-card purchases, checks, ACH transfers, bill payments, cash withdrawals, and fees. The available balance may differ from the ledger balance because of holds, pending transactions, and posting rules.
Financial Learning Cafe explains the subject. Your current agreement, official sources, facts, jurisdiction, and appropriately qualified professionals control the decision.